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当前位置: 首页 > 文章 > 溪黄草药材质量控制含量测定方法研究 中兽医医药杂志 2019 (6) 41-43
Position: Home > Articles > 溪黄草药材质量控制含量测定方法研究 Journal of Traditional Chinese Veterinary Medicine 2019 (6) 41-43

溪黄草药材质量控制含量测定方法研究

作  者：
马鹏文;师庆媛;朱昱澜;任一杰
关键词：
溪黄草;高效液相色谱法;齐墩果酸;熊果酸
摘  要：
目的:建立同时测定溪黄草药材中齐墩果酸、熊果酸含量的方法.方法:色谱柱为CAPCELL PAK C18MGⅡ(以十八烷基硅烷键和硅胶为填充柱),流动相为乙腈-甲醇-0.6%乙酸铵(67∶12∶21),流速为1 mL/min,检测波长为210 nm,柱温为30℃,进样量为20μL.结果:齐墩果酸和熊果酸分别在5.066~50.664μg/mL,9.735~97.352μg/mL范围内呈现良好的线性关系(r=0.998);精密度、稳定性、重复性试验的RSD<2.0%;平均回收率分别为99.78%(RSD=0.56%)和100.68%(RSD=0.67%).结论:该方法操作简单、重复性好、准确度高,可以用于溪黄草药材的质量控制.
作  者：
MA Pengwen;SHI Qingyuan;ZHU Yulan;REN Yijie;Gansu Key Laboratory of Traditional Chinese Medicine Solid Dispersible Preparation,Gansu Longshenrongfa Pharmaceutical Industry Co.,Ltd;Key Laboratory of Standard and Quality of Chinese Medicine Research of Gansu;
关键词：
Herba Rabdosiae Serrae;;high performance liquid chromatography;;oleanolic acid;;ursolic acid
摘  要：
Objective:To establish the method for simultaneous determination of oleanolic acid and ursolic acid in traditional Chinese medicine of Herba Rabdosiae Serrae. Methods:The chromatographic column was CAPCELL PAK C_(18) MG Ⅱ (with octadecylsilane chemically bonded silica as the packed column),and it was adopted with the mobile phase of acetonitrile-methanol-0.6% ammonium acetate(67∶12∶21). The detection wavelength was set at 210 nm with the flow rate of 1 mL·min~(-1),the column temperature of 30 ℃ and the injection volume of 20 μL. Results:Oleanolic acid and ursolic acid exhibited satisfied linear relationship in the range of 5.066 ～50.664 μg·mL~(-1) and 9.735 ～97.352 μg·mL~(-1),respectively(r =0.998).Meanwhile,the RSD of precision,stability and repeatability were less than 2.0%. The average recovery rates were 99.78%(RSD =0.56%) and 100.68%(RSD =0.67%), respectively. Conclusion: The proposed method of HPLC was simple,reproducible and accurate,and can be applied for quality control of medicinal material of Herba Rabdosiae Serrae.

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