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Position: Home > Articles > Isolation and Identification of High Efficient Sulfate-reducing Bacteria and Its Characteristics Journal of Agro-Environment Science 2008,27 (02) 210-213

高效硫酸盐还原菌的分离鉴定及其特性研究

作  者:
马保国;胡振琪;张明亮;陈胜华;王萍;黄晓燕
单  位:
中国矿业大学(北京)土地复垦与生态重建研究所
关键词:
硫酸盐还原菌;菌株分离;生理特性;硫酸盐还原
摘  要:
从煤矸石山淋溶水长期污染的黄土中分离获得一株硫酸盐还原菌。研究了菌株的生理生态特性,探讨了温度、pH和氧气对菌株的生长及转化硫酸盐效能的影响。结果表明,该菌株是革兰氏阳性菌,可以含有醇羟基的有机碳为碳源(电子供体),硫酸盐等为硫源(电子受体);在有氧和无氧条件下均能生长,菌株为兼性厌氧菌;生长的最适宜pH为7.16;最适生长温度为35℃;硫酸盐转化率最高达94.3%。同时测定了菌株的生长曲线。
译  名:
Isolation and Identification of High Efficient Sulfate-reducing Bacteria and Its Characteristics
作  者:
MA Bao-guo1,2,HU Zhen-qi1, ZHANG Ming-liang1, CHEN Sheng-hua1, WANG Ping1, HUANG Xiao-yan1 (1. Institute of Land Reclamation and Ecological Restoration, China University of Mining and Techonology, Beijing 100083, China; 2. College of Water Resource and Hydroelectricity , Hebei University of Engineering, Handan 056021,China)
关键词:
SRB; strain isolation; strain characteristics; sulfate reduction
摘  要:
Sulfate-reducing bacteria (SRB) is isolated from the loess polluted by acid mine drainage in coal mining aerrea in western China. The physiological characteristics of SRB’s physiology characteristics and the effect of environmental factors such as temperature, pH and O2 on sulfate reduction are studied. The results show that this strain of SRB is Gram-positive, and can grow with hydroxy organic carbon as electron donors and SO42- as electron acceptor under both anaerobic and aerobic conditions. The sulfate is reduced in this process and the rates of sulfate reduction reachs 94.3% .The optimum conditions for SRB growth are found to be at pH 7.16, 35 ℃. The growth curve of SRB is also identified in this paper.

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