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Position: Home > Articles > Determination of Three Medicinal Bioactive Components of Swertia franchetiana H.Smith Journal of Anhui Agricultural Sciences 2009,37 (4) 1623-1624

抱茎獐牙菜中3种药效成分的HPLC测定

作  者:
马玉花;纪兰菊;陈桂琛;卢学峰
单  位:
青海民族学院化学系;中国科学院西北高原生物研究所
关键词:
反相高效液相色谱法;抱茎獐牙菜;獐牙菜苦苷;龙胆苦苷;芒果苷
摘  要:
[目的]测定抱茎獐牙菜中不同部位的药效成分。[方法]采用反相高效液相色谱法,测定抱茎獐牙菜中不同部位獐牙菜苦苷、龙胆苦苷及芒果苷的含量。[结果]结果表明,HPLC法测定3种药效成分的最佳条件为流动相甲醇-0.02%磷酸水溶液,梯度洗脱,洗脱条件0~25 min,甲醇:20%~50%;流速1 ml/min,检测波长为260 nm。该方法具有很好的线性关系和回收率。[结论]抱茎獐牙菜根部龙胆苦苷的含量最高,其余2种药效成分在花的部位有较高的含量。
译  名:
Determination of Three Medicinal Bioactive Components of Swertia franchetiana H.Smith
作  者:
MA Yu-hua et al(Department of Chemistry,Qinghai Nationalities University,Xining,Qinghai 810007)
关键词:
Reversed-phase high performance liquid chromatography;Swertia franchetiana H.Smith;Swertiamarin;Gentiopicroside;Mangiferin
摘  要:
[Objective] The purpose was to determine three medicinal bioactive components in different parts of Swertia franchetiana H.Smith.[Method]The content of swertiamarin,gentiopicroside and mangiferin in Swertia franchetiana H.Smith were determined by reversed-phase high performance liquid chromatography.[Result]The optimum conditions of determining three medicinal bioactive components in different parts of Swertia franchetiana H.Smith were as follow: a Kromasil C18(250 mm×4.60 mm,5 μm);the mobile phase was composed of CH3OH and 0.02% phosphoric acid in water;the gradient condition was 0-25 min,CH3OH 20%-50%;the flow rate was 1 ml/min;the detection wavelength was 260 nm.The good linear relationships and recoveries were obtained under the optimum conditions.[Conclusion]The content of gentiopicroside is most high in the root,swertiamarin and mangiferin have higher content in the floral part of Swertia franchetiana H.Smith.

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