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Position: Home > Articles > Diversity of Actinomycetes in the Brewing Process of Luzhou-flavor Multiple-grains Liquor FOOD SCIENCE 2011 (23) 192-196

多粮浓香型白酒生产中放线菌的多样性

作  者：
张超;赵东;王涛;游玲;冯瑞章;王松;何建蓉
单  位：
宜宾学院生命科学与食品工程学院;五粮液集团技术中心;宜宾学院发酵工程与食品科学研究所;发酵资源与应用四川省高校重点实验室;宜宾红楼梦酒业股份有限公司
关键词：
浓香型白酒;放线菌;16S rRNA基因序列;系统发育分析;多样性
摘  要：
以改良的高氏I号培养基为基质,从多家宜宾多粮浓香型白酒生产企业的窖房空气、曲房空气、窖泥和糟醅分离到123株典型丝状放线菌,并对其进行了基于16S rRNA基因序列的系统发育分析。结果显示;123株典型放线菌分属于Streptomyce、Massilia、Nocardiopsis 3个属,Streptomyce为绝对优势属(121株,占总菌株数的98.4%);Streptomyce属的121株菌与该属内26个种的典型菌株序列相似度≥98%,其中,有45株与S.violascensISP 5183T序列最接近,分布于4个取样点;在4个取样点中,窖泥的丰富度指数和多样性指数最高,糟醅的优势度指数最高;各样点间放线菌的相似性较低,且都存在特有的放线菌类群。
译  名：
Diversity of Actinomycetes in the Brewing Process of Luzhou-flavor Multiple-grains Liquor
作  者：
ZHANG Chao1,2,3,ZHAO Dong4,WANG Tao1,2,3,YOU Ling2,3,FENG Rui-zhang1,2,WANG Song1,2,3,HE Jian-rong5(1.College of Life Science and Food Engineering,Yibin University,Yibin 644000,China;2.Key Laboratory of Fermentation Resource and Application of Institutes of Higher Learning in Sichuan,Yibin 644000,China;3.Fermentation Engineering and Food Science Institute,Yibin University,Yibin 644000,China;4.Technogical Center of Wuliangye Group Co.Ltd.,Yibin 644000,China;5.Hongloumeng Distillery Co.Ltd.,Yibin 644000,China)
关键词：
Luzhou-flavor multiple-grains liquor;actinomycetes;16S rRNA gene sequence;phylogenetic analysis;diversity
摘  要：
A total of 123 typical strains of actinomycetes were isolated from the air in fermentation workshop and Qu workshop,pit mud and fermented grains in several distilleries of Luzhou-flavor multiple-grains using modified Gogan-I medium.Their phylogenetic analysis was carried out based on 16S rRNA gene sequence.The results showed that the 123 strains belonged to 3 genera(Streptomyces,Massilia and Nocardiopsis),with Streptomyces being the dominant one(121 strains,accounting for 98.4% of the total isolated 123 strains).The 16S rRNA sequence similarity of the isolated 121 Streptomyces strains to those of the 26 typical strains published in Genbank was more than 98%,among which 45 strains were most closely related to the typical strains S.violascens ISP 5183T and distributed in 4 sample plots.Among the 4 sample plots,the richness index and diversity index was the highest in pit mud,and the dominance index was the highest in fermented grains.The similarity index among 4 sample plots was low and special species of actinomycetes existed in every plots.

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