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当前位置: 首页 > 文章 > 液体发酵条件对广东虫草菌丝生物量和胞外多糖产量的影响(英文) 食用菌学报 2012,19 (4) 59-66
Position: Home > Articles > Effects of Selected Parameters on Mycelial Biomass and Exopolysaccharide Production by Cordyceps guangdongensis Grown in Submerged Culture Acta Edulis Fungi 2012,19 (4) 59-66

液体发酵条件对广东虫草菌丝生物量和胞外多糖产量的影响(英文)

作  者：
林群英;黄浩;宋斌;李泰辉
单  位：
广东省微生物应用新技术公共实验室;广东省微生物研究所;广东省菌种保藏与应用重点实验室;广东省华南应用微生物重点实验室—省部共建国家重点实验室培育基地
关键词：
广东虫草;营养需求;环境参数;液体发酵
摘  要：
研究了液体发酵的温度、起始pH、碳源、氮源和碳氮比对广东虫草菌丝生物量和胞外多糖的影响。当发酵温度为25℃,培养基起始pH为7.0～9.0,麦芽膏和大豆浆粉分别为碳氮源时菌丝生物量最高;当发酵温度为28℃,培养基起始pH为5.0～7.0,葡萄糖和酵母膏为碳氮源时胞外多糖产量最高;以菌丝生物量和胞外多糖的产量为指标最优的碳氮比为12∶1。正交试验表明:以菌丝生物量为指标,优化的培养基为30g/L葡萄糖,30g/L麦芽膏,8g/L大豆浆粉和10g/L酵母膏;以胞外多糖产量为指标,优化的培养基为40g/L葡萄糖,30g/L麦芽膏,10g/L大豆浆粉和10g/L酵母膏。
译  名：
Effects of Selected Parameters on Mycelial Biomass and Exopolysaccharide Production by Cordyceps guangdongensis Grown in Submerged Culture
作  者：
LIN Qunying,HUANG Hao,SONG Bin,LI Taihui [ Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application,State Key Laboratory of Applied Microbiology(Ministry-Guangdong Province Joint Breeding Base),South China,Guangdong Open Laboratory of Applied Technology,Guangdong Institute of Microbiology,Guangzhou,Guangdong 510070,China ]
关键词：
Cordyceps guangdongensis;nutritional requirements;environmental parameters;submerged culture.
摘  要：
The effects of temperature,initial pH,carbon and nitrogen sources,and C:N ratio on mycelial biomass and exopolysaccharide(EPS) production by Cordyceps guangdongensis grown in submerged culture were evaluated.Mycelial growth was optimal at 25 ℃,an initial pH range from 7.0 to 9.0,and with malt extract and soybean steep powder as the carbon and nitrogen sources,respectively.EPS production was maximal at 28℃,an initial pH range from 5.0to 7.0,and with glucose and yeast extract as the carbon and nitrogen sources,respectively.Both biomass and EPS production were favored by a C:N ratio of 12:1.Orthogonal testing revealed the optimal medium for mycelial biomass production to consist of 30g/L glucose,30g/L malt extract,8g/L soybean steep powder and 10g/L yeast extract,and for EPS production to consist of 40g/L glucose,30g/L malt extract,10g/L soybean steep powder and 10g/L yeast extract.

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