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当前位置: 首页 > 文章 > 荧光分光光度法对黄酒氧自由基吸收能力检测方法的建立 食品科学 2013,34 (18) 189-192
Position: Home > Articles > Determination of Oxygen Radical Absorbance Capacity in Chinese Rice Wine by Fluorescence Spectrophotometry FOOD SCIENCE 2013,34 (18) 189-192

荧光分光光度法对黄酒氧自由基吸收能力检测方法的建立

作  者：
黄玥;陆文蔚;唐立伟;黄灵洁;白晨
单  位：
上海商学院旅游与食品学院
关键词：
黄酒;发酵酒;氧自由基吸收能力;荧光素;荧光分光光度法
摘  要：
目的:建立用荧光分光光度计测定黄酒氧自由基吸收能力(ORAC)的改进方法。方法:以市售黄酒为样品,使用荧光分光光度计,以荧光半数衰减时间为观察指标,通过Trolox标准曲线,换算得到黄酒的ORAC值,并测定最低检测限、重复性精密度、平行性精密度、加标回收率等。结果:激发波长为490nm,发射波长为517nm。相对荧光半数衰减时间与Trolox浓度的线性回归方程为Y=7.2357X-0.8189,线性决定系数R2为0.9948,检测范围0.28~8.00μmol/L,方法的重复性精密度为3.02%,平行性精密度为2.69%,加标回收率在93.00%~102.00%之间。检测样品的ORAC值为4.68~5.21μmol/mL。结论:ORAC荧光分光光度法简化了操作步骤,缩短了实验时间,同时保证了良好的准确度、精密度和稳定性,适用于测定不同品牌不同年份黄酒的ORAC。
译  名：
Determination of Oxygen Radical Absorbance Capacity in Chinese Rice Wine by Fluorescence Spectrophotometry
作  者：
HUANG Yue;LU Wen-wei;TANG Li-wei;HUANG Ling-jie;BAI Chen;Department of Tourism and Food Science,Shanghai Business School;
关键词：
Chinese rice wine;;fermented alcoholic beverage;;oxygen radical absorbance capacity(ORAC);;fluorescein(FL);;fluorescence spectrophotometry
摘  要：
Objective: To establish a modified method for the determination of oxygen radical absorbance capacity(ORAC) in Chinese rice wine by fluorescence spectrophotometry.Methods: Commercial samples of Chinese rice wine were determined by a fluorescence spectrophotometer for fluorescence half-decay time.As a result,a Trolox calibration curve was developed and used to calculate ORAC.The analytical method was validated with respect to limit of detection(LOD),precision for repeated and parallel determinations and spiked recovery.Results: The excitation wavelength was 490 nm,and emission wavelength was 517 nm.The regression equation between relative fluorescence half-decay time and Trolox concentration was Y = 7.2357X-0.8189 with R2of 0.9948 and detection range of 0.28–8.00 μmol Trolox/L.The precision for 5 repeated and 5 parallel determinations were 3.02% and 2.69%,respectively.The recovery rates of spiked samples were in the range of 93.00%–102.00%.The ORAC value of Chinese rice wine was 4.68–5.21 μmol Trolox/mL.Conclusion: Fluorescence spectrophotometry is a simple,time-saving,accurate,precise and stable method which is useful to determine ORAC in Chinese rice wines of different ages and from different brands

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