当前位置: 首页 > 文章 > 硝酸银对葡萄愈伤组织生长及其白藜芦醇含量的影响 甘肃农业大学学报 2008,43 (5) 65-68
Position: Home > Articles > Effect of silver nitrate on the multiplication of the callus from grapes cv.‘Pinot Noir’ and its resveratrol production Journal of Gansu Agricultural University 2008,43 (5) 65-68

硝酸银对葡萄愈伤组织生长及其白藜芦醇含量的影响

作  者:
张真;李胜;刘媛;吴兵;李婷;张青松;罗丽媛;薛冲
单  位:
甘肃农业大学生命科技学院植物组织培养研究室
关键词:
硝酸银;白藜芦醇;毒害;葡萄
摘  要:
以‘黑比诺’葡萄叶柄诱导并继代10个月的愈伤组织为试验材料,通过在培养基中(B5+1.0 mg/L KT)分别添加0、1、5、10、20、30 mg/L不同浓度的AgNO3,研究了AgNO3对愈伤组织生长和白藜芦醇累积的效应,结果表明,除5 mg/L AgNO3外,其他几种浓度处理均对愈伤组织的生长产生抑制作用;添加10 mg/L的AgNO3对愈伤组织中白藜芦醇的累积效果最佳,含量达17.884μg/g,高出对照3.6倍;但AgNO3浓度过高(30 mg/L)时,白藜芦醇含量明显下降;当浓度为5 mg/L时,白藜芦醇的产量最大,为18.878μg/瓶,是对照的4.7倍.
译  名:
Effect of silver nitrate on the multiplication of the callus from grapes cv.‘Pinot Noir’ and its resveratrol production
作  者:
ZHANG Zhen,LI Sheng,LIU Yuan,WU Bing,LI Ting,ZHANG Qing-song,LUO Li-yuan,XUE Chong(College of Life Science and Technology,Gansu Agricultural University,Lanzhou 730070,China)
关键词:
silver nitrate;resveratrol;poison;grapes
摘  要:
The subcultured callus induced from grapes cv'Pinot Noir'stem was used as the experimental material in vitro,and the effect of silver nitrate stress with different concentration at 0,1,5,10,20 and 30 mg/L on the callus multiplication and resveratrol production were studied in this paper.The results showed that silver nitrate at different concentrations inhabited the growth of the callus except treatment at 5 mg/L.Adding 10 mg/L silver nitrate promoted the accumulation of resveratrol,the content reached 17.884 μg/g,3.6 times higher than that of the control.Resveratrol production reached the maximum with 18.878 μg/flask when silver nitrate was added at 5 mg/L,4.7 times more than the control.

相似文章

计量
文章访问数: 10
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊