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当前位置: 首页 > 文章 > 硝酸银对根癌农杆菌介导的木薯遗传转化的影响 生物技术通报 2013,0 (3) 166-170
Position: Home > Articles > Effect of Silver Nitrate on Agrobacterium Mediated Genetic Transformation of Cassava Biotechnology Bulletin 2013,0 (3) 166-170

硝酸银对根癌农杆菌介导的木薯遗传转化的影响

作  者：
郭运玲;尹奇;孔华;黄启星;左娇;贺立卡;郭安平
单  位：
中国热带农业科学院热带生物技术研究所农业部热带作物生物学与遗传资源利用重点实验室
关键词：
硝酸银;木薯;遗传转化;不定芽诱导
摘  要：
旨在提高木薯遗传转化效率,以成熟培养10-15 d的华南木薯SC8品种的胚状体子叶为外植体,研究硝酸银在根癌农杆菌介导的木薯遗传转化中的影响。结果表明,硝酸银对木薯SC8胚状体子叶愈伤形成有明显的抑制作用,对不定芽的分化有显著的促进作用。硝酸银的浓度在2-8 mg/L,对未经农杆菌处理的SC8胚状体子叶不定芽的分化率从58.5%提高到87.5%;愈伤组织形成率从92%下降到15.3%。对经农杆菌处理的SC8胚状体子叶不定芽的分化率从55.3%提高到85.3%;愈伤组织形成率从87.5%下降到8.5%。同时发现,经农杆菌转化后不定芽诱导所需硝酸银的最适浓度高于未经农杆菌处理的不定芽诱导所需硝酸银最适浓度,前者为8 mg/L,后者为6 mg/L。硝酸银对木薯SC8的遗传转化有促进作用。
译  名：
Effect of Silver Nitrate on Agrobacterium Mediated Genetic Transformation of Cassava
作  者：
Guo Yunling Yin Qi Kong Hua Huang Qixing Zuo Jiao He Lika Guo Anping(Key Laboratory of Tropical Crops,Ministry of Agriculture,Institute of Tropical Bioscience and Biotechnology CATAS,Haikou 571101)
关键词：
Silver nitrate Cassava Genetic transformation Adventitious buds induction
摘  要：
It was to improve the conversion efficiency of cassava genetic transformation.The effect of silver nitrate on the Agrobacterium mediated genetic transformation of cassava was assessed by the maturation of 10-15 days of Southern China cassava SC8 embryoid cotyledon as explants.The results showed that silver nitrate could improve the regeneration frequency and reduce callus formation in SC8.The use of silver nitrate at concentrations between 2 and 8 mg/L increased shoot organogenesis frequency from 58.5% to 87.5% while callus formation decreased from 92% to 15.3% without the Agrobacterium cocultivation;and shoot organogenesis frequency increased from 55.3% to 85.3% while callus formation decreased from 87.5% to 8.5% with Agrobacterium cocultivation.Meantime,the silver nitrate optimum concentration of adventitious buds induction by the Agrobacteriummediated genetic transformation was higher than that without the Agrobacteriumtreatment,the former was 8 mg/L,the latter was 6 mg/L.The application of silver nitrate can stimulate cassava SC8 genetic transformation.

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