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当前位置: 首页 > 文章 > 三种抗原检测H_1N_1猪流感病毒抗体的比较 广东畜牧兽医科技 2014,39 (1) 20-24
Position: Home > Articles > Comparation of Three Methods for Detection of Antibodies to H_1N_1 Swine Influenza Virus Guangdong Journal of Animal and Veterinary Science 2014,39 (1) 20-24

三种抗原检测H_1N_1猪流感病毒抗体的比较

作  者：
杨倩;薛春宜;朱道中;李晓明;曹永长
单  位：
广东出入境检验检疫局检验检疫技术中心;中山大学生命科学学院有害生物控制与资源利用国家重点实验室
关键词：
昆虫杆状病毒表达系统;猪流感病毒;类病毒脂质体;抗体检测
摘  要：
本研究采用昆虫杆状病毒表达系统制备了H1N1亚型猪流感病毒的HA蛋白、类病毒脂质体、病毒样颗粒,分别作为抗原进行包被,采用间接酶联免疫吸附实验(ELISA)检测H1N1猪流感病毒抗体。特异性实验结果表明,单独表达的HA蛋白和类病毒脂质体特异性良好,但是病毒样颗粒不能区分不同亚型的流感病毒;敏感性实验结果表明,类病毒脂质体作为抗原的敏感性最好;重复性实验结果表明,三种抗原的重复性好,ELISA实验的批间和批内变异系数均小于10%;稳定性实验结果表明,在4℃可至少稳定保存1年。综上所述,类病毒脂质体有较高的免疫反应性,与灭活全病毒相比有更好的安全性,在抗体水平测定方面有较好的应用前景。
译  名：
Comparation of Three Methods for Detection of Antibodies to H_1N_1 Swine Influenza Virus
作  者：
Yang Qian;Xue Chunyi;Zhu Daozhong;Li Xiaoming;Cao Yongchang;State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University;Technical Center,Guangdong Entry-Exit Inspection and Quarantine Bureau;
关键词：
Bac-to-Bac baculovirus expression system;;swine influenza virus;;virus-like liposome;;antibody detection
摘  要：
HA protein, virus-like liposomes, and virus-like particles, expressed by Bac-to-Bac baculovirus expression system, were coated as antigens to detect the HIN1 swine influenza virus antibody using indirect enzyme-linked immunosorbent assay(ELISA). The results of specificity experiments showed that the single-expressed HA protein and virus-like liposomes had good specificity, but the virus-like particles could not distinguish different subtypes of influenza virus. The results of sensitivity experiments showed that virus-like liposomes had the best sensitivity. The results of repetitive experiments showed that the repetitiveness of the three methods was high, and the coefficient of variation within-run and between-run were less than 1%. The results of stability experiments showed that three antigens could be stable at 4 ℃ for at least one year. In a word, the virus-like liposome has strongest immunoreactivity, and is safer than the inactivated whole influenza virus. Thus it represents a promising method in detection of H1N1 antibodies.

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