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Position: Home > Articles > RP-HPLC Studies on Quantitative Determination of Carnosic Acid in Rosemarinus officinalis L FOOD SCIENCE 2005,26 (4) 203-205

反相液相色谱法测定迷迭香中鼠尾草酸的含量

作  者:
梁振益;黄广民;张德拉;刘长生;陈雷;陈宗宪
单  位:
海南大学理工学院;海南万恒生物工程有限公司
关键词:
反相高效液相色谱;迷迭香;鼠尾草酸
摘  要:
建立反相高效液相色谱法分离和测定迷迭香中鼠尾草酸的含量。以Hil sil C18反相键合硅胶色谱柱(250mm×4.6mm,5μm),乙腈+甲醇(85:15)为流动相,流速1.0ml/min,检测波长为210nm,外标法定量。鼠尾草酸在20~200μg/ml范围有良好的线性关系,r=0.9998,回收率为98.6%。本方法准确、可靠、简便,可用于迷迭香中鼠尾草酸的含量测定。
译  名:
RP-HPLC Studies on Quantitative Determination of Carnosic Acid in Rosemarinus officinalis L
作  者:
LIANG Zhen-yi1,HUANG Guang-min1,ZHANG De-la1, LIU Chang-sheng1,CHEN Lei1,CHEN Zong-xian2 (1.College of Science and Engineering, Hainan University, Haikou 570228, China; 2.Hainan Everlasting Biological Engineering Co.Ltd., Haikou 570125, China)
关键词:
RP-HPLC;Rosemarinus officinalis L;Carnosic acid
摘  要:
To establish a HPLC method for the separation and determination of Carnosic acid in Rosemarinus officimalis L, the Pheromenex C18 reverse Phase column (250mm×4.6mm, 5μm) and acetonitrile-methanol as mobile phase were used with flow rate 1.0ml/min detecting spectrum 210 nm and quantitating value by external standard method. The standard curve of linear relationship was of 20~200μg/ml and r=0.9998.The average recovery was 98.6%. The established method was accurate, sensitive, simple and reliable for the determination of Carnosic acid in Rosemarinus officinalis L.

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