中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 酿酒酵母组成型表达甜叶菊糖基转移酶UGT76G1及相关性质研究 食品工业科技 2015,36 (13) 162-165+170
Position: Home > Articles > Constitutive expression and characteristics of glycosyltransferase UGT76G1 in Saccharomyces cerevisiae Science and Technology of Food Industry 2015,36 (13) 162-165+170

酿酒酵母组成型表达甜叶菊糖基转移酶UGT76G1及相关性质研究

作  者：
王钰;陈量量;杜婷;李艳;严明;郝宁;许琳
单  位：
南京工业大学生物与制药工程学院
关键词：
酿酒酵母;组成型启动子;糖基转移酶UGT76G1;全细胞催化;莱鲍迪甙A
摘  要：
本研究将酿酒酵母穿梭质粒p ESC-Leu的诱导型启动子GAL1和GAL10分别替换为PGK1和TEF1启动子,构建了组成型双启动子表达载体p ESCD,再将甜叶菊糖基转移酶UGT76G1的编码基因亚克隆到p ESCD的Sal I和Xho I酶切位点之间,构建了组成型表达UGT76G1的重组质粒p ESCD-UGT。将p ESCD-UGT转化到酿酒酵母YPH499中进行表达,结果确定该重组酵母在SD-L液体培养基培养24h达到稳定期,菌体培养8h蛋白表达量高,选用1%的甲苯作为重组菌全细胞催化的通透剂时,催化15h产生288mg/L的莱鲍迪甙A,其产量是对照组的5倍。
译  名：
Constitutive expression and characteristics of glycosyltransferase UGT76G1 in Saccharomyces cerevisiae
作  者：
WANG Yu;CHEN Liang-liang;DU Ting;LI Yan;YAN Ming;HAO Ning;XU Lin;College of Biotechnology and Pharmaceutical Engineering,Nanjing Tech University;
关键词：
Saccharomyces cerevisiae;;constitutive promoter;;glycosyltransferase UGT76G1;;whole cell transformation;;rebaudioside A
摘  要：
In this study,a new expression vector p ESCD which contains the two constitutive promoters,p PGK1 and p TEF1 that replaced the original induced promoter GAL1 / GAL10 in p ESC- Leu,was designed and constructed.The synthetic glycosyltransferase UGT76G1 coding gene was inserted into the restriction sites,Sal I and Xho I of p ESCD to construct the recombinant plasmid p ESCD- UGT,which was then transformed into the Saccharomyces cerevisiae strain YPH499.The results confirmed that the recombinant cells grew into the stable phase when cultured for 24 h and the highest expression of the recombinant protein was observed when cultured for 8h in SD- L.In case of 1% methylbenzence as the cell permeating agent,288 mg / L RA was produced after reaction for 15 h using the whole- cell catalyst,which was 5 times higher than that of thecontrol group.

相似文章

导出引用
计量
文章访问数: 10
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46