单 位：
广东海洋大学食品科技学院广东省水产品加工与安全重点实验室水产品深加工广东普通高等学校重点实验室
关键词：
罗非鱼肉;脱脂豆粕;共沉淀蛋白;蛋白得率
摘 要：
以罗非鱼肉和脱脂豆粕为原料,采用p H调节法制备罗非鱼-大豆共沉淀蛋白(Co-p),探讨溶解p H、不同质量比混合的原料、溶解时间对可溶性蛋白得率的影响及沉淀p H对蛋白沉淀得率的影响。结果表明,p H调节法回收罗非鱼-豆粕共沉淀蛋白的最佳酸溶p H2.0、3.0,碱溶p H11.0、12.0,原料比1∶1,溶解时间30 min;SDS-PAGE分析显示,可溶性共沉淀蛋白条带深/浅(极端p H2.0蛋白降解),表明可溶性蛋白含量高/低,从分子量分布范围可知,共沉淀蛋白主要由肌球蛋白重链、7 S抗原蛋白的三个亚基、肌动蛋白、肌球蛋白轻链、11 S抗原蛋白的两个亚基和小分子水溶性蛋白组成,表示可溶性共沉淀蛋白组成齐全;酸/碱可溶性共沉淀蛋白最佳沉淀p H为4.5,在此条件下,溶解、沉淀过程的蛋白得率分别为88.05%~94.70%。经冷冻干燥得到共沉淀蛋白粉即Co-p(1∶1),其蛋白含量高于85%,脂肪含量在0.84%左右,灰分含量低于4.17%;可用p H调节法回收罗非鱼-豆粕共沉淀蛋白。
译 名：
Extraction process and composition analysis of co-precipitated protein from tilapia muscle and soybean meal
作 者：
QI Hui-hong;ZHOU Chun-xia;ZHU Pan-hong;LI Ting;HONG Peng-zhi;College of Food Science and Technology,Guangdong Ocean University,Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety,Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution;
关键词：
tilapia muscle;;defatted soybean meal;;co-precipitate protein;;protein yield
摘 要：
Co-precipitation protein was prepared from Tilapia muscle and defatted soybean meal by p H-shifting.Effect of p H value,mixing ratios of tilapia to defatted soybean meal,dissolving time on soluble protein yield was determined in the extraction process.And then effect of p H value on precipitated protein yield was examined as well.The results showed that the solubility of co-precipitation protein was best under the conditions of dissolution that the optimal p H value was 2.0,3.0,11.0,and 12.0,the tilapia to defatted soybean meal 1 ∶ 1,and the dissolving time of 30 min. The SDS-PAGE results showed that soluble co-precipitation protein bands of deep/shallow showed that the content of soluble co-precipitation protein of high/low( protein degradation at p H2.0).According to the distribution of molecular weight,it was found that the co-precipitation protein consisted mainly of myosin heavy chain,actin,myosin light chain,small molecule water soluble protein,three subunits of the antigen protein of 7 S and two subunits of the antigen protein of 11 S,which showed that the composition of soluble co-precipitation protein was complete.The precipitation condition of soluble co-precipitation protein was p H4.5.In the above four conditions,the recovered protein yields from tilapia muscle and soybean meal were 88.05% ~ 94.70% by acid/alkaline solubilization and precipitation processing.The co-precipitated protein powder,Co-p( 1 ∶ 1),was obtained by freeze-drying. The protein content was above 85%,the fat content was about 0.84%,and the ash content was less than 4.17%.The use of p H-shifting method was beneficial for the recovery of co-precipitated protein.
