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Position: Home > Articles > Optimization of Cellulase Produced by Mixed Solid Culture Journal of Henan Agricultural Sciences 2014,43 (10) 150-155

混菌固态发酵产纤维素酶条件的优化

作  者:
李蕾;张力;乔梁;史兆国
单  位:
江苏农牧科技职业学院;甘肃农业大学
关键词:
混菌固态发酵;纤维素酶;绿色木霉;黑曲霉
摘  要:
以羧甲基纤维素酶活力、滤纸酶活力为考察指标,探讨绿色木霉(Trichoderma viride)化L4C和黑曲霉(Aspergillus niger)混合固态发酵产纤维素酶的最佳培养时间;并以降解率为考察指标,通过单因素和正交试验对混菌固态发酵产纤维素酶条件进行优化,以促进秸秆资源的饲料化利用、缓解粮食危机,降低环境污染。结果表明,绿色木霉化L4C和黑曲霉混合固态发酵最佳时间为3d,最佳总接种量为10%,绿色木霉化L4C与黑曲霉的最佳接种比例为1∶1,培养基最佳氮源为硫酸铵,麸皮与稻草秸秆粉最佳质量比为3∶7,最适宜培养基含水量为50%。在该条件下稻草秸秆半纤维素降解率可达34.83%,纤维素降解率可达39.75%,木质素降解率可达27.41%。
译  名:
Optimization of Cellulase Produced by Mixed Solid Culture
作  者:
LI Lei;ZHANG Li;QIAO Liang;SHI Zhao-guo;Gansu Agricultural University;Jiangsu Agri-animal Husbandry Vocational College;
关键词:
solid fermentation of mixed bacteria;;cellulase;;Trichoderma viride;;Aspergillus niger
摘  要:
The optimum fermentation time of solid fermentation of mixed Trichoderma viride L4 C and Aspergillus niger with filter paper activity and carboxymethyl cellulase activity as indexes was studied,and the solid fermentation conditions were optimized by single factor test and orthogonal experiments with degradation rate as index,so as to promote the utilization of straw for fodder,relieving food crisis,reducing the environmental pollution.The optimum solid fermentation conditions were fermentation time of 3d,total inoculum volume of 10%,inoculation ratio of Trichoderma viride L4 Cto Aspergillus niger of 1∶1,(NH4)2SO4as nitrogen source,mass ratio of bran to straw stalk powder of 3∶7,moisture contents of 50%.Under the above conditions,the degradation rate of hemicellulose,cellulose,lignin for straw stalk were 34.83%,39.75% and 27.41%.

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