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当前位置: 首页 > 文章 > 四氢嘧啶提高脂肪酶催化合成油酸乙酯产率的研究 食品工业科技 2010,31 (11) 224-227
Position: Home > Articles > Ectoine improving yield of ethyl oleate catalyzed by lipase Science and Technology of Food Industry 2010,31 (11) 224-227

四氢嘧啶提高脂肪酶催化合成油酸乙酯产率的研究

作  者：
王越;张苓花
单  位：
大连海事大学环境科学与工程学院;大连工业大学生物工程学院
关键词：
四氢嘧啶;油酸乙酯;脂肪酶;无溶剂体系
摘  要：
为了对抗乙醇对酶的竞争性抑制作用,提高酶法合成油酸乙酯的酯化率,在米曲霉DM-01全细胞脂肪酶催化合成油酸乙酯的无溶剂体系中添加补偿性溶质。结果表明,添加补偿性溶质四氢嘧啶效果最好,四氢嘧啶浓度为1.5mmol/L时,0.1g全细胞脂肪酶催化油酸乙酯酯化合成反应48h,油酸乙酯酯化率为61.4%,与未添加四氢嘧啶的相比,提高了24.8%,酯化速率提高了38.0%。在全细胞脂肪酶3次重复使用反应中添加四氢嘧啶,与未加四氢嘧啶的比较,油酸乙酯相对酯化率分别提高了24.7%、33.3%和166.8%。添加四氢嘧啶为提高酶法合成油酸乙酯的产率提供了一种新手段。
译  名：
Ectoine improving yield of ethyl oleate catalyzed by lipase
作  者：
WANG Yue1,2,ZHANG Ling-hua1 (1. Environmental Science and Engineering College,Dalian Maritime University,Dalian 116026,China; 2. College of Biotechnology,Dalian Polytechnic University,Dalian 116034,China)
关键词：
ectoine;ethyl oleate;lipase;solvent-free system
摘  要：
To resist the competitive alcohol inhibition on lipase,improving the esterification degree of ethyl oleate by enzymatic synthesis,compatible solutes were added to the solvent-free synthesis system catalyzed by whole-cell lipase from Aspergillus oryzae DM-01. The results indicated that the addition of ectoine led to an increase in the synthesis of ethyl oleate. When 0. 1g whole-cell lipase was added in the synthesis system,and the reaction was conducted for 48h,the esterification degree of ethyl oleate reached a maximum of 61. 4% in the presence of 1. 5mmol/L ectoine,an increase of 24. 8% compared with that in the absence of ectoine. The esterification rate increased by 38. 0%. Ectoine was added into synthesis systems for whole-cell lipase reuse during three recycling tests. Relative esterification degrees of ethyl oleate increased by 24. 7%,33. 3% and 166. 8% compared with the control without ectoine. The supplementation of ectoine provided a new method for the purpose of improving yield of ethyl oleate catalyzed by enzyme.

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