当前位置: 首页 > 文章 > 高产色素红曲菌的筛选鉴定及其液态发酵条件优化 福建农业学报 2016,V31 (4) 424-430
Position: Home > Articles > Screening and Identifying a High Pigment-producing Monacus Strain,and Optimizing Its Liquid Fermentation Fujian Journal of Agricultural Sciences 2016,V31 (4) 424-430

高产色素红曲菌的筛选鉴定及其液态发酵条件优化

作  者:
贾瑞博;周文斌;陈竟豪;李燕;刘凯丽;赵慧;刘斌;吕旭聪
单  位:
国家菌草工程技术研究中心;福建农林大学食品科学学院
关键词:
色素;红曲菌;筛选;鉴定;液态发酵;优化
摘  要:
从福建永春、安溪、古田、南平、三明、龙岩等地收集的红曲米中分离筛选得到了1株高产色素红曲菌L03,利用形态学和分子生物学的方法对其进行鉴定,结果表明为紫色红曲菌Monascus purpureus。同时通过单因素和正交优化试验确定了其最佳的液态发酵条件:以8%的甘油为碳源、1.5%蛋白胨为氮源、硫酸镁0.1%、磷酸二氢钾0.25%、接种量6%、发酵温度32℃、时间9d、摇床转速180r·min-1、250mL容器中装液量100mL,在此液态发酵条件下其总色价平均值为1 578U·mL-1。
译  名:
Screening and Identifying a High Pigment-producing Monacus Strain,and Optimizing Its Liquid Fermentation
作  者:
JIA Rui-bo;ZHOU Wen-bin;CHEN Jing-hao;LI Yan;LIU Kai-li;ZHAO Hui;LIU Bin;L Xu-cong;National Engineering Research Center of JUNCAO Technology;College of Food Science,Fujian Agriculture and Forestry University;Sunshine International Group Technology Development CO.;
关键词:
red pigment;;Monascus;;selection;;identification;;liquid fermentation;;optimization
摘  要:
The Monascus strain,L03,was isolated from the red kojic rice samples collected from Yongchun,Anxi,Gutian,Nanping,Sanming,and Longyan in Fujian for its high red-pigment-producing ability.Based on the morphology and molecular biology of L03,it was identified to be M.purpureus.Subsequently,single factor and orthogonal experiments were conducted to optimize the conditions for the liquid fermentation of the yeast.Color of the fermented suspension reached 1,578U/mL when the yeast was inoculated 6% by weight into a liquid medium consisting of 8% of glycerol,1.5 % of peptone,0.1% of Mg2SO4,and 0.25% of KH2PO4 in a loading volume of100mL/250 mL and incubated at 32℃ with constant agitation at the speed of 180 rpm for 9days.

相似文章

计量
文章访问数: 11
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊