当前位置: 首页 > 文章 > 反相高效液相色谱法测定辐花肋柱花中苦龙苷含量的研究 安徽农业科学 2007,35 (4) 33-34
Position: Home > Articles > Determination of Amarogerth in Lomatogonium Rotatum by RP-HPLC Journal of Anhui Agricultural Sciences 2007,35 (4) 33-34

反相高效液相色谱法测定辐花肋柱花中苦龙苷含量的研究

作  者:
俞青芬;林鹏程
单  位:
青海民族学院药学系;青海民族学院化学系
关键词:
反相高效液相色谱;苦龙苷;辐花肋柱花;龙胆科
摘  要:
建立了反相高效液相色谱法测定辐花肋柱花中苦龙苷的含量。采用Kromasil-C18(250 mm×4.6 mm,5μm)的色谱柱,以甲醇-水(52∶48,体积比)为流动相等度洗脱,流速0.8 ml/min,检测波长254 nm,柱温30℃。结果表明,在试验条件下苦龙苷与其他成分达到基线分离。苦龙苷的线性范围为0.028-0.165μg(r=0.9995)。该方法简便、快速,结果准确、可靠。
译  名:
Determination of Amarogerth in Lomatogonium Rotatum by RP-HPLC
作  者:
YU Qing-fen et al (Chemistry Department,Qinghai Institute of Nationalities,Xining,Qinghai 810007)
关键词:
RP-HPLC;Amarogerth;Lomatogonium rotatum;Gentianaceae
摘  要:
In this article,RP-HPLC was applied to determine the content of amarogerth in Lomatogonium rotatum.The sample were separated on the column of Kromasil-C18(250 mm×4.6 mm,5 μm) which eluted with methanol and water(0.04% phosphoric acid).The ratio of methanol and water was 52∶48 with detected wavelength at 254 nm,flow rate at 0.8 ml/min,column temperature at 30 ℃.The result showed that the compound was mostly separated.The linear ranges of amarogerth was 0.028~0.165 μg(r=0.999 5).This method was rapid and accurate.

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