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当前位置: 首页 > 文章 > 嗜热链球菌CH9胞外多糖的分离纯化及结构分析 食品科学 2012,33 (19) 107-112
Position: Home > Articles > Purification and Structural Characterization of Exopolysaccharide Produced by Streptococcus the rmophilus CH9 FOOD SCIENCE 2012,33 (19) 107-112

嗜热链球菌CH9胞外多糖的分离纯化及结构分析

作  者：
张新源;刘会平;曹春玲;季玲
单  位：
天津科技大学食品工程与生物技术学院教育部食品营养与安全重点实验室
关键词：
嗜热链球菌;胞外多糖;分离纯化;结构分析
摘  要：
对嗜热链球菌CH9胞外多糖进行分离纯化,经DEAE-52离子交换柱及Sephadex G-100凝胶柱得到单一组分,利用高效液相色谱(HPLC)测其分子质量,结果表明:EPS1a为中性多糖,分子质量为1.8×106u;EPS2a、EPS3a为酸性多糖,分子质量分别为1.06×106、1.05×106u。经琼脂糖凝胶电泳及Sephadex G-100凝胶柱鉴定3种糖均为单一组分。紫外及红外光谱扫描结果显示:3种糖均具有多糖的特征吸收峰,且EPS2a、EPS3a可能为糖与蛋白的复合物。刚果红实验表明:EPS1a具有三股螺旋结构,而其他两种糖并未发现此现象。β消去实验证明:EPS1a、EPS3a为—O—连接,EPS2a为—N—连接。
译  名：
Purification and Structural Characterization of Exopolysaccharide Produced by Streptococcus the rmophilus CH9
作  者：
ZHANG Xin-yuan,LIU Hui-ping,CAO Chun-ling,JI Ling(Key Laboratory of Food Nutrition and Safety,Ministry of Education,School of Food Engineering and Biological Technology,Tianjin University of Science and Technology,Tianjin 300457,China)
关键词：
Streptococcus thermophilus CH9;exopolysaccharides;purification;structural characterization
摘  要：
Crude exopolysaccharides(EPS) isolated from the fermentation supernatant of Streptococcus thermophilus CH9 were fractionated by DEAE-52 column chromatography into EPS1,EPS2 and EPS3.Sephadex G-100 column chromatography was used to further fractionate the three factions to harvest their respective major components named as EPS1a,EPS2a and EPS3a,respectively.EPS1a was a neutral polysaccharide with a molecular weight of 1.8 × 106u,and EPS2a and EPS3a were acidic polysaccharides with a molecular weight of 1.06 × 106 u and 1.05 × 106 u,respectively.Agarose gel electrophoresis analysis indicated that all these three polysaccharides consisted of a single component.UV and IR spectroscopic analyses showed that all of them had characteristic adsorption peaks of polysaccharide.EPS2a and EPS3a might be polysaccharideprotein complexes.Congo red test revealed that EPS1a rather than EPS2a and EPS3a had a three-stranded helix structure.Beta elimination test demonstrated that EPS1a and EPS3a were-O-linked,but EPS2a was a-N-linked polysaccharide.

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