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Position: Home > Articles > Isolation,Purification and Anti-complement Activity of Water-soluble Polysaccharides from Chaenomeles cathayensis FOOD SCIENCE 2008,29 (5) 120-124

野木瓜水溶性多糖的分离纯化及抗补体活性研究

作  者：
王文平;郭祀远;李琳;孙黔云;唐维媛
单  位：
华南理工大学轻工与食品学院;贵州省发酵工程与生物制药重点实验室;中国科学院贵州省天然产物化学重点实验室
关键词：
野木瓜;多糖;分离;纯化;抗补体活性
摘  要：
采用水提醇沉工艺获得了水溶性野木瓜粗多糖(CCP),经过Sevag法脱蛋白,大孔树脂脱色及透析,得到野木瓜精多糖,再经DEAE-纤维素和Sepharose Cl-6B柱层析,从野木瓜中分离得到多糖组分CCP1,经过高效凝胶渗透色谱法(HPGPC)鉴定为均一组分,测定其相对分子量(MW)为2.879×106D。高效液相法测定CCP1的单糖组成为:鼠李糖、阿拉伯糖、果糖、甘露糖、葡萄糖,其摩尔比为0.034:0.228:0.045:0.055:0.638。补体结合实验表明,多糖CCP1在体外具有一定抗补体活性,并呈一定的剂量效应关系。
译  名：
Isolation,Purification and Anti-complement Activity of Water-soluble Polysaccharides from Chaenomeles cathayensis
作  者：
WANG Wen-ping1,GUO Si-yuan1,LI Lin1,SUN Qian-yun2,TANG Wei-yuan3(1.College of Light Industry and Food Sciences,South China University of Technology,Guangzhou 510640,China;2.Key Laboratory of Chemistry for Nature Products of Guizhou Province,Chinese Academy of Sciences,Guiyang 550002,China;3.Guizhou Provincial Key Laboratory of Fermentation Engineering and Biological Pharmacy,Guiyang 550003,China)
关键词：
Chaenomeles cathayensis;polysaccharide;isolation;purification;anti-complement activity
摘  要：
The crude water-soluble polysaccharides from Chaenomeles cathayensis fruits were isolated with hot water extraction followed by ethanol precipitation.The polysaccharides were deproteinized using Sevag method,decolorized by macroporous resin and then dialyzed.The fine water-soluble Chaenomeles cathayensis polysaccharides(CCP) were obtained and further fraction-ated to elute for the component CCP1 by anion exchange column chromatography on DEAE-cellulose.The purity of CCP1 was examined by gel filtration chromatography of Sepharose Cl-6B.The purified polysaccharide CCP1 was proved to be homoge-neous by HPGPC and its molecular weight(MW) was 2.879×106 D.Analysis of monosaccharide composition by HPLC indicated that CCP1 consists of rhamnose,arabinose,fructose,mannitose,glucose and their molar ratio is 0.034:0.228:0.045:0.055:0.638.The main fraction CCP1 was subjected to the complement-fixation experiment.CCP1 expressed some anti-complementary activity in vitro and dosage-effect relationship between them existing.

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