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当前位置: 首页 > 文章 > Dot-ELISA检测兔疥螨抗体方法的建立和应用 动物医学进展 2009,30 (4) 12-17
Position: Home > Articles > Establishment and Application of Dot-ELISA for Detecting Antibodies against Sarcoptes scabiei var.cuniculi Progress in Veterinary Medicine 2009,30 (4) 12-17

Dot-ELISA检测兔疥螨抗体方法的建立和应用

作  者：
郝桂英;杨光友;古小彬
单  位：
四川农业大学动物医学院
关键词：
斑点酶联免疫吸附试验;兔疥螨;抗体检测
摘  要：
将从病兔痂皮内收集的疥螨经研磨、冻融、离心后,制成可溶性抗原,作为诊断抗原,建立Dot-ELISA方法检测兔疥螨血清抗体。研究确定了该方法的最佳工作条件。制备的诊断膜片特异性强,不与兔瘟病毒、兔大肠埃希菌、兔附红细胞体等阳性血清反应。膜片具有良好的灵敏性,高免血清作1∶210稀释亦能检出;重复性试验表明该法重复性良好。诊断膜片在4℃保存5个月其检测活性不变。结果表明,建立的Dot-ELISA可用于免疥螨抗体的检测。
译  名：
Establishment and Application of Dot-ELISA for Detecting Antibodies against Sarcoptes scabiei var.cuniculi
作  者：
HAO Gui-ying1,2,YANG Guang-you1,GU Xiao-bin1(1.College of Veterinary Medicine,Sichuan Agricultural University,Ya'an,Sichuan,625014,China;2.Department of Animal Science,Xichang College,Xichang,Sichuan,615013,China)scabieivar cuniculi.
关键词：
Dot-ELISA;Sarcoptes scabiei var.cuniculi;antibody detection
摘  要：
The soluble proteins of sarcoptes scabiei var.cuniculi were extracted by repeating freezing thaw,grinding and centrifugation,which was selected as diaphragm-carried antigen in Dot-ELISA.Through repeated experiments,the optimal trial conditions of Dot-ELISA were determined.The specificity was proved by the blocking and cross-reaction tests.The diaphragm did not react with the antibodies against RHDV,E.coli Eperythrozoon and fungi.The diaphragm had the ability to detect the positive serum when it was diluted to 1∶210 and so it had good sensitivity.The repeatability test proved that the method or the diagnostic diaphragm was stable.Stored at 4 ℃ for at least 5 months,the diagnostic diaphragm's sensitivity and specificity didn't change,so it had good stability.Results have shown that Dot-ELISA can be used to defect sarcoptes

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