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应用斑点酶联免疫吸附试验快速检测饲料中的沙门氏菌

作  者:
雷风;韩志辉;张红见
单  位:
青海大学农牧学院
关键词:
斑点酶联免疫吸附试验;检测;成品饲料;沙门氏菌
摘  要:
选用硝酸纤维素(N C)膜作固相载体,建立检测沙门氏菌的斑点酶联免疫吸附试验诊断方法,检测成品饲料100份,对沙门氏菌的最低检出量为400个/m L,沙门氏菌阳性检出率43.00%(43/100),常规分离培养阳性检出率为38.00%(38/100),两者符合率为83.72%,差异不显著(P>0.05)。试验表明该方法简便、特异、快速、结果直观,便于在基层推广使用。
译  名:
Checking Salmonella in Animal Feed by Dot-ELISA
作  者:
LeiFeng,Han Zhihui,Zhang Hongjian(Faculty of Agriculture and Animal Sciences,Qinghai University,Xining 810003)
关键词:
Dot-ELISA;Checking;Animalfeed;Salmonella
摘  要:
A Dot-enzyme linked immunosorbentassay was successfully developed by using nitrocellulose membrane as solid carrier,100 anim alfeeds were checked,the results showed thatthe m inim um was 400 salm onella/m L,and the positive rate ofsalm onella was 43.00%(43/ 100).the positive rate was 38.00%(38/100) identified by the technology of routine isolation and culture.positive coincidence rate of both m ethods was 83.72% and there was no significantdifference(P>0.05).the trialindicated thatthis m ethod was sim ple,special,quick,directand easy extention and application atprim ary level.

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