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当前位置: 首页 > 文章 > 鲍鱼内脏多糖分离纯化与抗氧化活性评价 南方农业学报 2019,50 (2) 372-377
Position: Home > Articles > Isolation and purification of visceral polysaccharides from abalone and its antioxidant activity Journal of Southern Agriculture 2019,50 (2) 372-377

鲍鱼内脏多糖分离纯化与抗氧化活性评价

作  者：
陈胜军;刘先进;杨贤庆;李来好;黄卉;吴燕燕;胡晓;李春生
单  位：
中国水产科学研究院南海水产研究所/农业农村部水产品加工重点实验室/国家水产品加工技术研发中心;上海海洋大学食品学院
关键词：
鲍鱼内脏;分离纯化;多糖;抗氧化
摘  要：
【目的】分离纯化鲍鱼内脏多糖(Abalone viscera polysaccharide,AVP),并评价其抗氧化活性,为AVP的高值化利用提供参考依据。【方法】以鲍鱼内脏为原料,采用酶法提取并初步纯化出AVP,测定其DPPH自由基清除率、羟自由基(OH自由基)清除率和还原力3个抗氧化指标,评价AVP抗氧化能力;同时用葡聚糖凝胶G-100柱层析分离AVP,并对分离出的组分进行抗氧化能力比较。【结果】AVP质量浓度在1～10 mg/mL时,DPPH自由基清除率线性方程为y=9.715x+1.0182(R~2=0.9958),半抑制浓度(IC_(50))为5.04 mg/mL;OH自由基清除率线性方程为y=6.2778x-8.5275(R~2=0.9603),IC_(50)为9.32 mg/mL;还原力线性方程为y=0.0782x+0.0045(R2=0.9989),A_(700,0.2)为2.50 mg/mL。AVP具有一定的抗氧化能力,但与抗坏血酸(Vc)相比,其抗氧化能力较弱。AVP经葡聚糖凝胶G-100柱层析可分离出两个组分(AVP1和AVP2),AVP1和AVP2均具有一定的抗氧化活性,且AVP1抗氧化能力强于AVP2。【结论】通过葡聚糖凝胶柱层析可从AVP中有效分离出AVP1和AVP2两个组分,其中AVP1的抗氧化能力更强,在抗氧化添加剂及保健品等领域具有较好的开发利用前景。
译  名：
Isolation and purification of visceral polysaccharides from abalone and its antioxidant activity
作  者：
CHEN Sheng-jun;LIU Xian-jin;YANG Xian-qing;LI Lai-hao;HUANG Hui;WU Yan-yan;HU Xiao;LI Chun-sheng;South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences/Key Laboratory of Aquatic Product Processing,Ministry of Agriculture and Rural Affairs/National R & D Center for Aquatic Product Processing;College of Food Science and Technology,Shanghai Ocean University;
关键词：
abalone viscera;;separation and purification;;polysaccharide;;antioxidation
摘  要：
【Objective】Abalone viscera polysaccharide(AVP)was isolated and purified,then its antioxidant activity was evaluated. It provided technical support for high-value utilization of AVP.【Method】In this research,the abalone viscera was used as experimental material to extract and purify AVP by enzymatic extraction and preliminary purification.The three antioxidant indexes,namely DPPH radical scavenging rate,hydroxyl radical(OH radical)scavenging rate and reducing power of AVP were determined,and the antioxidant capacity of AVP was evaluated. Meanwhile,the AVP was isolated by glucose gel G-100 column layer and the antioxidant abilities of isolated fractions were studied and compared.【Result】The experimental results showed that the mass concentration of AVP was in the range of 1-10 mg/mL,and the linear equation of DPPH radical scavenging rate was y=9.715 x+1.0182(R~2=0.9958),half-inhibitory concentration(IC_(50))value was 5.04 mg/mL. The linear equation of OH radical scavenging rate was y=6.2778 x-8.5275(R~2=0.9603),IC_(50) value was9.32 mg/mL. The linear equation of reducing power was y=0.0782 x+0.0045(R~2=0.9989),and the value of A_(700,0.2) was 2.50 mg/mL. AVP could be separated into two components(AVP1 and AVP2)by G-100 column chromatography,both AVP1 and AVP2 had certain antioxidant activity,and AVP1 had higher antioxidant capacity than AVP2.【Conclusion】AVP could be separated into AVP1 and AVP2 by sephadex column chromatography. AVP1 had higher antioxidant activity and therefore enjoys a good prospect of development and utilization in the fields of antioxidant additive and health products.

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