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Position: Home > Articles > Characteristics of endogenous proteases from ablone( Haliotis Discus Hannai Ino) viscera Science and Technology of Food Industry 2016,37 (19) 81-85

鲍鱼内脏内源蛋白酶特性的研究

作  者：
李梅娟;刘丹;贾真;陈小艺;方婷;陈锦权
单  位：
福建农林大学食品科学学院
关键词：
鲍鱼内脏;内源性蛋白酶;酶活性;相对分子量
摘  要：
本文研究了鲍鱼内脏内源蛋白酶(内源酶)的性质。分别探讨了p H、温度、金属离子和特异性蛋白酶抑制剂对内源酶活性的影响。结果表明内源酶蛋白质含量为125.10 mg/m L。钠离子在浓度(0~1.36 mol/L)范围明显促进内源酶活性,钙离子有稳定酶活作用,对酶活无显著影响,而高浓度的钠离子,铜离子和锌离子会抑制酶活。内源酶在最适p H4左右和p H7左右,有较高的酶活和很好的热稳定性,且能被胃蛋白酶抑制剂(Pepstain)、丝氨酸蛋白酶抑制剂(PMSF)、胰蛋白酶抑制剂(SBTI)和胰凝乳蛋白酶抑制剂(TPCK)抑制。本研究可为缩短传统制作鲍鱼酱油发酵时间提供理论依据。
译  名：
Characteristics of endogenous proteases from ablone( Haliotis Discus Hannai Ino) viscera
作  者：
LI Mei-juan;LIU Dan;JIA Zhen;CHEN Xiao-yi;FANG Ting;CHEN Jin-quan;Fujian Agriculture and Forestry University,College of Food Science;
关键词：
abalone viscera;;endogenous protease;;protease activity;;relative molecular weight
摘  要：
To research the characteristics of endogenous proteases from abalone( Haliotis Discus Hannai Ino)viscera.The effects of p H,temperature,metal ions and specific protease inhibitors on endogenous proteases activity were investigated. The results showed that the content of endogenous proteases was 125.10 mg / m L. The protease activity could be increased at low concentrations( 0 ~ 1.36 mol / L) of Na~+,and the activity of protease tended to be stable under the presence of Ca~(2+),however,high concentrations of Na~+,Cu~(2+) and Zn~(2+) significantly inhibited the activity of protease.The optimum working p H of endogenous proteases were located at about 4 and 7at which condition protease showed high activity and good thermal stability. Endogenous proteases could be inhibited by Pepstain,PMSF( phenyl- methyl- sulphonyl- fluoride),SBTI( soybean trypsin inhibitor) and TPCK( tosyl- phenylalanine chloromethyl- ketone).The research can provide theoretical basis for shortening the traditional fermentation time of abalone sauce.

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