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Position: Home > Articles > Study on TTC Method for Testing Seed Viability of Asarum heterotropoides Fr.Schmidt var.mandshuricum(Maxim.) Kitag. Special Wild Economic Animal and Plant Research 2016,38 (3) 31-35

应用TTC法快速测定北细辛种子生活力

作  者:
邢丽伟;于营;欧阳艳飞;张新静;朴向民;张佳琪;郭靖
单  位:
中国农业科学院特产研究所;松原市久千草药业有限公司
关键词:
北细辛;2,3,5-氯化三苯基四氮唑法;种子生活力
摘  要:
研究北细辛种子生活力的快速测定方法,为北细辛种子利用及安全保存提供技术支撑。采用2,3,5-氯化三苯基四氮唑(TTC)染色法,设置不同的浸种时间、TTC浓度、染色温度、染色时间的正交试验,对北细辛种子生活力进行测定。影响北细辛种子生活力测定值大小的因素依次为TTC浓度>染色温度>染色时间>浸种时间;最佳条件:温水浸种6h、0.1%TTC溶液、30℃避光染色18h;最佳反应条件下测得种子生活力与发芽率实测值很相近。TTC法可作为北细辛种子生活力测定的标准方法,对北细辛栽培及种质资源保存具有重要意义。
译  名:
Study on TTC Method for Testing Seed Viability of Asarum heterotropoides Fr.Schmidt var.mandshuricum(Maxim.) Kitag.
作  者:
XING Li-wei;YU Ying;OUYANG Yan-fei;ZHANG Xin-jing;PIAO Xiang-min;ZHANG Jia-qi;GUO Jing;Institute of Special Wild Economic Animals and Plants,Chinese Academy of Agricultural Science;Songyuan Jiuqiancao Pharmaceutical Company Limited;
关键词:
Asarum heterotropoides F;;Schmidt var;;mandshuricum(Maxim;;) Kitag;;TTC Method;;seed viability
摘  要:
The aim of the study is to study an appropriate method for testing seed viability of Asarum heterotropoides Fr.Schmidt var.mandshuricum(Maxim.) Kitag.Using the 2,3,5-triphenyl tetrazolium chloride(TTC) method,the orthogonal test design set different soaking time,different TTC concentration,different dyeing temperature,and different dyeing time were detected to research seed viability measurement conditions.The optimum conditions for testing seed viability were soaking seeds for 6h and then staining in 0.1% TTC solution at 30℃ for 18 h.Under these conditions seed viability is significantly related to germination.Research the optimum conditions for testing seed viability,It has great significance in cultivation and germplasm conservation.TTC method could be used as the standard method for testing seed viability.

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