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Position: Home > Articles > Analysis of Fatty Acids in Spirulina by Gas Chromatography-Mass Spectrometry FOOD SCIENCE 2009,30 (22) 280-282

气相色谱-质谱法分析螺旋藻中脂肪酸

作  者:
许华;潘灿平
单  位:
中国农业大学理学院
关键词:
螺旋藻;脂肪酸组成;气相色谱-质谱法(GC-MS)
摘  要:
建立用毛细管柱气相色谱-质谱法(GC-MS)分析螺旋藻中脂肪酸组成及亚麻酸含量的方法。螺旋藻中的脂肪酸用质谱进行定性确证分析,螺旋藻的脂肪酸组成用总离子流色谱图(TIC)归一化法计算。同时通过TIC使用外标法对螺旋藻中的亚麻酸含量进行定量分析。质谱定性结果表明所检螺旋藻中含有10种不饱和脂肪酸,根据峰面积归一法不饱和脂肪酸占46.774%,而γ-亚麻酸含量高达26.702%。GC-MS定量分析螺旋藻中亚麻酸在50.0~5000.0μg/ml质量浓度范围内有较好的线性关系,线性方程为Y=15843X-533217,R2=0.9986。定量分析结果表明所检螺旋藻中两种亚麻酸总量为396mg/100g,而γ-亚麻酸为393mg/100g。
译  名:
Analysis of Fatty Acids in Spirulina by Gas Chromatography-Mass Spectrometry
作  者:
XU Hua,PAN Can-ping(College of Science,China Agricultural University,Beijing 100094)
关键词:
Spirulina;fatty acid;gas chromatography-mass spectrometry(GC-MS)
摘  要:
A gas chromatography-mass spectrometry(GC-MS) method was established to identify compositions of fatty acids and determine the content ofγ-linolenic acid in Spirulina.The compositions of fatty acids in Spirulina were identified through MS and γ-linolenic acid was quantitatively determined by a normalization method of total ion chromatograph.Qualitative analysis results revealed that 10 unsaturated fatty acids were detected and the content of unsaturated fatty acids was 46.774%;meanwhile,the content ofγ-linolenic acid was 26.702%.The determination ofγ-linolenic acid using GC-MS was expressed to be Y = 15843X-533217 with a correlation coefficient of 0.9986.An excellent linear range forγ-linolenic acid was 50.0-5000.0 μg/ml.Total content of linolenic acid in Spirulina was 396 mg/100 g andγ-linolenic acid was 393 mg/100 g.

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