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Position: Home > Articles > Optimization of Mixotrophic Medium for Spirulina Culture by Response Surface Methodology FOOD SCIENCE 2009,30 (7) 109-113

螺旋藻混合营养培养基响应面法的优化研究

作  者：
谯顺彬;迟海洋;张奕婷;董汝晶;张义明
单  位：
贵州科技工程职业学院;贵州省发酵工程与生物制药重点实验室;广东食品药品职业学院
关键词：
螺旋藻;单因子试验;因子分析设计;响应面法;藻体干重
摘  要：
本实验利用自制的光生物反应器进行螺旋藻混合营养培养,分别采用单因子试验和因子分析设计对添加的混合营养成分进行筛选。结果表明,影响螺旋藻生长的主要因子是葡萄糖、硝酸铵和VB1。利用响应面法(RSM)对上述三个显著因子的最佳水平范围进行研究,并建立以藻体干重(DW)为响应值,以葡萄糖、硝酸铵和VB1为自变量的二次多项式数学模型。最后对光照强度和营养成分的不同补加周期进行优化研究,最终藻体干重为1.822g/L。
译  名：
Optimization of Mixotrophic Medium for Spirulina Culture by Response Surface Methodology
作  者：
QIAO Shun-bin1,CHI Hai-yang2,ZHANG Yi-ting3,DONG Ru-jing1,4,ZHANG Yi-ming1,3, (1.Guizhou Technological Engineering and Vocational College, Guiyang 550008, China; 2.Guangdong Food and Drug Vocational College, Guangzhou 510520, China; 3.Guizhou Province Key Laboratory of Fermentation Engineering and Biopharmacy, Guiyang 550003, China; 4.College of Chemical Engineering of Guizhou University, Guiyang 550003, China)
关键词：
Spirulina;single-factor test;factional factorial design;response surface methodology;dry weight
摘  要：
In this study, a self-made bioreactor was employed for mixotrophic culture of Spirulina. The important factors influencing growth of Spirulina were screened out by single-factor test and factional factorial design, which are added concentrations of glucose, NH4NO3 and VB1. The response surface methodology was used to optimize the above three factors. Based on the results of optimization test, a quadratic regression equation was built up with dry weight as the response value and with added concentrations of glucose, NH4NO3 and VB1 as independent variables. Finally, the light intensity and the added cycles of glucose, NH4NO3 and VB1 were optimized. Under the optimized conditions, the dry weight of Spirulina is up to 1.822 g/L.

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