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当前位置: 首页 > 文章 > 细胞冻融辅助热水浸提螺旋藻多糖及脱蛋白工艺优化 食品科学 2012,33 (24) 111-116
Position: Home > Articles > Optimization of Polysaccharide Extraction from Spirulina platensis by Cell Freeze-Thaw Cooperated with Hot Water Extraction and Deproteinization FOOD SCIENCE 2012,33 (24) 111-116

细胞冻融辅助热水浸提螺旋藻多糖及脱蛋白工艺优化

作  者：
朱劼;任淑振;彭江晨
单  位：
常州大学制药与生命科学学院;江苏省溧阳天目湖保健品有限公司
关键词：
螺旋藻;多糖;细胞冻融;热水浸提;脱蛋白
摘  要：
以螺旋藻粉为原料,将细胞冻融技术与热水浸提技术相结合提取藻多糖。通过单因素及正交试验,对冻融后破碎细胞残余物中剩余多糖的提取及Sevag法脱除蛋白进行研究,优化其工艺条件,并与传统的热水浸提工艺对比。结果表明,在料液比1:16、浸提温度80℃、浸提时间1.5h的条件下热水浸提破碎细胞残余物,效果最佳,与细胞上清液合并后的多糖总提取率达到6.64%,高于传统的热水浸提法近14%;而采用Sevag法脱除蛋白的最佳工艺条件为烷醇比5:1、料液试剂比3:1、处理次数3次。在此条件下,蛋白脱除率及多糖损失率分别为81.4%和17.3%。粗多糖质量进一步分析得,总糖含量85.3%,多糖分子质量84700u,多分散度1.306;而传统热水浸提工艺得到的多糖分子质量为54800u,多分散度2.007,其粗多糖产品质量低于细胞冻融辅助热水浸提工艺得到的产品质量。
译  名：
Optimization of Polysaccharide Extraction from Spirulina platensis by Cell Freeze-Thaw Cooperated with Hot Water Extraction and Deproteinization
作  者：
ZHU Jie1,REN Shu-zhen1,PENG Jiang-chen2(1.School of Pharmaceutical Engineering and Life Science,Changzhou University,Changzhou 213164,China;2.Liyang Tianmuhu Health Product Company Ltd.,Changzhou 213300,China)
关键词：
Spirulina platensis;polysaccharide;cell freeze-thaw;hot water extraction;deproteinization
摘  要：
Polysaccharide was extracted from Spirulina platensis by cell freeze-thaw cooperated with hot water extraction.Operating parameters for hot water extraction and deproteinization by Sevag method of polysaccharide from cell debris after cell disruption were optimized using an orthogonal array design.The best results were obtained after extraction at 80 ℃ for 1.5 h at a solid-to-solvent ratio of 1:16(g/mL).The extract obtained was pooled with the supernatant after cell disruption and a total total polysaccharide yield of 6.64% was obtained,which was approximately 14% higher than that obtained by hot water extraction alone.The optimal conditions for deprotenizing crude polysaccharide by Sevag’s method were found to be three treatment cycles with a mixture of chloroform and n-butyl alcohol(5:1,V/V) at a solid-to-solvent ratio of 1:3(g/mL).Under these conditions,the deprotentinizaiton rate and the polysaccharide loss were 81.4% and 17.3%,respectively.The crude polysaccharide extract had a total sugar content of 85.3% and contained an 84700 u polysaccharide with a ploydispersity of 1.306,whereas a 54800 u polysaccharide with a ploydispersity of 2.007 was obtained by hot water extraction alone.

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