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当前位置: 首页 > 文章 > 氢化物原子荧光法分析不同地区刺参总硒和无机硒含量及分布规律 食品科学 2015,36 (12) 113-118
Position: Home > Articles > Analysis of Total Selenium and Inorganic Selenium in Apostichopus japonicus from Different Regions by Hydride Generation-Atomic Fluorescence Spectrometry FOOD SCIENCE 2015,36 (12) 113-118

氢化物原子荧光法分析不同地区刺参总硒和无机硒含量及分布规律

作  者：
李秀梅;孙国华;杨建敏;任利华;马元庆;谷伟丽;张健;刘兆存
单  位：
上海海洋大学水产与生命学院;山东华春渔业有限公司;山东省海洋资源与环境研究院
关键词：
氢化物原子荧光光谱法;刺参;总硒;无机硒;有机硒
摘  要：
为测定我国黄渤海海域刺参群体硒含量并探索其分布规律,采用氢化物原子荧光光谱法进行含量分析。结果显示,呼吸树为总硒和无机硒含量最高的组织,平均值分别为(14.171±3.412)mg/kg和(6.194±3.829)mg/kg,不同组织内的总硒、无机硒含量变化呈现一致的规律,硒含量由高到低为:呼吸树>消化道>肌肉>内壁>外壁,同时刺参体壁和肌肉(食用部分)中有机硒比例在75.6%~89.4%之间(除东营地区刺参群体肌肉组织的有机硒比例最低为53.6%);5个地区刺参群体同一组织内的总硒及无机硒含量有显著性差异,东营地区呼吸树、消化道和肌肉中无机硒含量较高。
译  名：
Analysis of Total Selenium and Inorganic Selenium in Apostichopus japonicus from Different Regions by Hydride Generation-Atomic Fluorescence Spectrometry
作  者：
LI Xiumei;SUN Guohua;YANG Jianmin;REN Lihua;MA Yuanqing;GU Weili;ZHANG Jian;LIU Zhaocun;College of Fisheries and Life Science, Shanghai Ocean University;Shandong Provincial Key Laboratory of Restoration for Marine Ecology, Shandong Marine Resource and Environment Research Institute;Shandong Huachun Fisheries Co. Ltd.;
关键词：
hydride generation-atomic fluorescence spectrometry(HG-AFS);;Apostichopus japonicus;;total selenium;;inorganic selenium;;organic selenium
摘  要：
This study aimed to explore the selenium status of Apostichopus japonicus from the Yellow Sea and the Bohai Sea. Total selenium and inorganic selenium were detected by hydride generation-atomic fluorescence spectrometry(HGAFS). This method had a wide linear dynamic range and good precision and accuracy, so it was suitable for the determination of different forms of selenium in aquatic products. Selenium levels in four populations of Apostichopus japonicus from Dalian, Dongying, Yantai, Qingdao, and Rizhao respectively were examined. Among five tissues investigated, respiratory tree contained the highest levels of organic selenium and inorganic selenium which were averaged at(14.171 ± 3.412) and(6.194 ± 3.829) mg/kg, respectively, and both of which followed the same decreased order of respiratory tree > digestive tract > muscle > inner body wall > outer body wall. The proportions of organic selenium in body wall and muscle(the edible part) were between 75.6% and 89.4%, except for Apostichopus japonicas muscle from Dongying containing the lowest level(53.6%) of organic selenium. There were significant differences in the contents of total selenium and inorganic selenium in the same tissues from different regions, and the contents of inorganic selenium in respiratory tree, digestive tract and muscle were higher in Dongying population than in those from other regions.

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