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当前位置: 首页 > 文章 > 三七总皂苷的乙醇超声法提取工艺及急性毒性研究 安徽农业科学 2008,36 (21) 255-256
Position: Home > Articles > Study on the Extraction Process of Total Saponins from Panax notognseng by Ethanol Ultrasonic Method and its Acute Toxicity Journal of Anhui Agricultural Sciences 2008,36 (21) 255-256

三七总皂苷的乙醇超声法提取工艺及急性毒性研究

作  者：
陈玉勇;秦枫;刘靖;朱海燕
单  位：
江苏牧医学院食品科技系;江苏省动物药品工程技术研究开发中心
关键词：
三七总皂苷;超声波提取;急性毒性试验
摘  要：
[目的]优化三七总皂苷的乙醇超声法提取工艺。[方法]选取乙醇浓度、料液比、提取温度3个因素进行正交试验,优化三七总皂苷的乙醇超声法提取工艺,并研究提取产品的急性毒性。[结果]各因素对三七总皂苷提取率的影响由大到小依次为:提取温度>乙醇浓度>料液比,最佳提取工艺为:12倍液的50%乙醇浸泡三七粉末12h后,40℃下超声提取50min后过滤,滤渣再次提取,合并滤液;滤液经减压干燥、除杂、浓缩后得精制三七总皂苷粉末,其最大耐受剂量(MTD)大于20.0g/kg体重,属无毒级。[结论]与传统方法相比,乙醇超声波法提取三七总皂苷节省溶剂,降低能耗,提高效率,所得产品为无毒产品。
译  名：
Study on the Extraction Process of Total Saponins from Panax notognseng by Ethanol Ultrasonic Method and its Acute Toxicity
作  者：
CHEN Yu-yong et al (Department of Food Science and Technology,Jiangsu College of Animal Husbandry and Veterinary,Taizhou,Jiangsu 225300)
关键词：
Total saponins of Panax notognseng;Ultrasonic extraction;Acute toxicity test
摘  要：
[Objective] The study aimed to optimized the extraction process of total saponins from Panax notognseng.[Method] The orthogonal test with 3 factors of ethanol concn.,solid-liquid ratio and extracting temperature was conducted to optimize the extraction process of total saponins from P.notognseng by ethanol ultrasonic method and study the acute toxicity of the extracted product.[Result] The effect of each factor on the yield of total saponins from P.notognseng in order was extracting temperature>ethanol concn.>ratio of solid and liquid.The optimum extracting process was as follows:the powder of P.notoginseng was dipped in 50% ethanol solution diluted at 12 times for 12 h and extracted with ultrasonic at 40 ℃ for 50 minutes;after being filtrated the filter residue was extracted again;the two filtrates were combined and refined into the powder of total saponins from P.notognseng after decompression drying,impurity removal and concentration,which had MTD>20.0 g/kg BW and belonged to non-toxic class.[Conclusion] Compared with the traditional extracting method,the ethanol ultrasonic method for extracting total saponins from P.notognseng could save solvent,decrease energy consumption and increase extracting efficiency and the extract was a non-toxic product.

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