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当前位置: 首页 > 文章 > 甘草次酸在Caeo-2细胞模型中的转运机制 东北林业大学学报 2011,39 (12) 100-103
Position: Home > Articles > Transport Mechanism of Glycyrrhetinic Acid in Human Intestinal Epithelial Caco-2 Cells Journal of Northeast Forestry University 2011,39 (12) 100-103

甘草次酸在Caeo-2细胞模型中的转运机制

作  者：
王筱萌;祖元刚;王微;付玉杰;张琳
关键词：
甘草次酸;Caco-2细胞模型;双向转运;P-糖蛋白;抑制剂;Glycyrrhetinic acid; Caco-2 cell model; Bidirectional transport; P-glycoprotein; P-glycoprotein inhibitors
摘  要：
采用液相-质谱检测甘草次酸在HBSS磷酸盐缓冲液中的转运量,结果表明:甘草次酸的转运量受时间、浓度以及P-糖蛋白抑制剂维拉帕米和汉防己甲素的影响,表观渗透系数P(appBL-AP)为(17.1±4.2)*10^-6-(54.3±6.3)x10^-6cm·s^-1、P(appAP-BL)为(15.6±3.4)*10^-6cm·s^-1;甘草次酸在小肠内的吸收主要是被动扩散,口服吸收较好并且存在P-糖蛋白的外排机制.
译  名：
Transport Mechanism of Glycyrrhetinic Acid in Human Intestinal Epithelial Caco-2 Cells
摘  要：
An experiment was conducted to determine the transferred amount of glycyrrhetinie acid in phosphate buffered solution by HPLC-MS/MS, in order to study the transport mechanism of glycyrrhetinic acid with Caco-2 cell monolayers. Results showed that the transferred amount of glycyrrhetinic acid was affected by culture time, drug concentration and P-glycoprorein inhibitor verapamil and Tetrandrine. The apparent permeability coefficients of glycyrrhetinic acid were ( 17.1 ±4.2 ) *10^-6 ( 54.3±6.3 ) * 10^4 cm· s^-1 for PappBL-AP, and (15.6±3.4) *10^-4 cm · s^-1 for PappAP-BL, and the bidirectional transport characteristics were different. It indicated that both active transport and passive diffusion contributed to the ab- sorption of glycyrrhetinic acid in Caco-2 cell monolayers, but the passive diffusion was the dominant process. The P-glycoprotein may play an important role in the effiux of glycyrrhetinic acid.

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