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Position: Home > Articles > Cloning and Bioinformatics Analysis of cDNA of β-catenin Gene in Peking Duck Chinese Journal of Animal Science 2009,45 (5) 5-9

北京鸭β-catenin基因cDNA的克隆和生物信息学分析

作  者：
王功帅;侯水生;黄苇;李花妮;高玉鹏
单  位：
中国农业科学院北京畜牧兽医研究所动物营养学国家重点实验室
关键词：
β-catenin;cDNA;克隆;生物信息学;北京鸭
摘  要：
试验探讨了北京鸭β-catenin基因的生物学功能,为进一步研究该基因对鸭皮肤毛囊的影响奠定基础。以北京鸭胚胎皮肤组织为材料,根据GenBank中发表的鸡、鹅等物种的β-catenin基因序列,设计并合成4对引物,采用RT-PCR方法,克隆北京鸭β-catenincDNA,并运用DNAMAN软件及在线工具对所得到的序列进行生物信息学分析。结果表明:北京鸭β-catenin基因cDNA长度为2996bp(Genbank登录号为FJ169885),包含由2346个碱基组成的编码区(CDs),有起始密码子ATG和终止密码子TAA,编码781个氨基酸;北京鸭β-catenin基因核苷酸序列与鸡、鹅、中华鳖、人和家猪相应区段(CDs)的同源性分别为95.06%、94.12%、90.11%、84.83%、84.31%;其氨基酸序列与鸡、鹅、中华鳖、猪、人的氨基酸同源性达99%以上,表明在进化关系上,β-catenin氨基酸序列有很高的保守性。
译  名：
Cloning and Bioinformatics Analysis of cDNA of β-catenin Gene in Peking Duck
作  者：
WANG Gong-shuai1,2, HOU Shui-sheng2, HUANG Wei2, LI Hua-ni1,2, GAO Yu-peng1* (1.College of Animal Science and Technology, Northwest A & F University, Shanxi Yangling 712100, China; 2.State Key Laboratory of Animal Nutrition Science, Institute of Animal Science, CAAS, Beijing 100193, China)
关键词：
β-catenin gene; cDNA; cloning; bioinformatics; Peking duck
摘  要：
This paper discussed the biological function β-catenin gene of the Peking duck, which was a basis to study Peking duck hair follicle. Four pairs of primers were designed according to β-catenin gene sequence the sequence of the chicken and goose published in GenBank ,and combined bioinformatics and RT-PCR technique to amplify the β-catenin gene of Peking duck. The cDNA sequence of Peking duck β-catenin gene was analyzed by using the DNAMAN software and online tool. The results showed that sequence of cDNA of the Peking duck β-catenin gene was 2 996 bp (GenBank Accession No. FJ169885) contained a coding sequence (CDs) of 2 346 bp which encoded a 781 amino acid including ATG start codon and TAA stop codon. The cloned Peking duck β -catenin cDNA coding sequence had an overall similarity with a comparable region of chicken (95.06% ), goose (94.12% ), Chinese softshell turtle (90.11% ), pig (84.83%), human (84.31%). Phylogenetic tree of amino acid analysis indicated that the amino acid sequence of β-catenin protein of the Peking duck is highly 99% with chicken, goose, Chinese softshell turtle, pig and human.

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